COMBINATION OF TRANS-CINNAMALDEHYDE AND PIPERINE AGAINST COMPLETE FREUND'S ADJUVANT INDUCED ARTHRITIS ANIMAL MODEL ILLUSTRATING ANTI-ARTHRITIC ACTIVITY.
Swapnil Jadhav* and Kedar Prabhavalkar
ABSTRACT
An autoimmune disease, rheumatoid arthritis (RA) causes chronic inflammation of the joints characterized by hyperplasia of synovial cells and angiogenesis in the affected joints, which eventually leads to the erosion of cartilage and bone. The purpose of the present study was to examine anti-arthritic activity of trans-cinnamaldehyde (3mg/kg) and piperine (8mg/kg) combination using in vivo Complete Freund‟s Adjuvant (CFA) induced arthritis animal model in male Wistar rats. Paw volume and percentage inhibition of paw edema in CFA-injected and non-injected paws, arthritic index, and body weight measurement were estimated and conclusions from histopathological analysis were drawn. The study involves induction of arthritis to rats of all groups using CFA, followed by subsequent treatment with piperine (15mg/kg), trans-cinnamaldehyde (5mg/kg) and {piperine (8mg/kg) + trans-cinnamaldehyde (3mg/kg)} combination respectively. Indomethacin is used as a reference standard. The (piperine + trans-cinnamaldehyde) combination significantly reduce the swelling in the CFA-injected paws and inhibited the inflammation in non-injected sites and also, inhibited the activation and proliferation of T-cells thereby inhibiting the secondary lesions. Additionally, the histopathological studies support and justify the anti-arthritic effect of (piperine + trans-cinnamladehyde) combination. Thus, the (piperine + trans-cinnamaldehyde) combination showed significant anti-arthritic activity and the results were comparable with the standard drug indomethacin.
Keywords: Rheumatoid arthritis, Complete Fruend's Adjuvant, Trans-cinnamaldehyde, Piperine, Indomethacin.
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