PHYTOCHEMICAL SCREENING, GC-MS, LC-MS ASSISTED METABOLITE PROFILING, AND ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF NATURAL PRODUCTS FROM MEDICINAL PLANT VITEX NEGUNDO
Aziz Alans*, Amat Al-Qader F. Al-Anesi, Ali Alrabie, Mazahar Farooqui and Deepak D. Kayande
ABSTRACT
This study aimed to assess the biological activities, including antioxidant and antibacterial characteristics, of a methanol extract of Vitex negundo leaves, as well as their phytochemical composition, using chromatographic techniques. The results of the GC-MS and LC-MS analyses showed that the methanol extract was stable. The extract was also tested for its antioxidant and antibac erial activities in vitro. With 90 peaks in the LC-MS study, the methanolic extract contained 96 distinct phytochemicals; in contrast, the GC-MS analysis revealed 32 compounds in the extract. The methanolic extract showed potent antibacterial action against A. aeroginosa, S. aureus, and S. pyogenes, with a minimum inhibitory concentration (MIC) of 125 μg/mL. When tested with DPPH, a strong antioxidant response was seen. This is the first study that we are aware of that details the chemical makeup of Vitex negundo as well as its antioxidant and antibacterial capabilities. Extract from Vitex negundo may have potential as an antibacterial and natural antioxidant in food preservation and human health, according to the current study's findings.
Keywords: Vitex negundo, LC-MS, GC-MS analysis, Antioxidant activity, Antibacterial activity
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