European Journal of Biomedical and Pharmaceutical Sciences

PHYTOCHEMICAL APPROACH AND RESEARCH INTO THE ANTIOXIDANT ACTIVITY OF THE ROOTS AND STEMS OF CASSIA SIEBERIANA DC. : A MEDICINAL PLANT FROM COTE D'IVOIRE

N’Guessan Patrick Audrey*, Mouho Guéi Didier Roselin, Ouattara Logopho Hyacinthe, Koné Songui Issouf,
Zon Doumadé and Kablan Ahmont Landry Claude

ABSTRACT

This work involved phytochemical investigations and research into the antioxidant activity of Cassia sieberiana
root and stem extracts. This study aims to justify the traditional use of C. sieberiana in the traditional treatment of
certain diseases linked to oxidative stress. To achieve this, hydroethanolic crude extracts obtained by maceration
and selective extracts obtained by liquid-liquid contact were prepared. The selective extracts were used for
phytocompound identification and antioxidant activity research by TLC, while the hydroethanolic crude extracts
were used for tube identification tests, UPLC-MS analysis, phytophenol determination and research into their
antioxidant potential. Phytochemical sorting of specialized metabolites using color tube reactions and TLC
revealed the presence of polyphenols, flavonoids, tannins, phenolic acids, sterols, terpenes and saponins in both
plant organs. UPLC-MS analysis detected a flavonoid in the roots. The assays showed that total phenolics, total
flavonoids and total condensed tannins were more concentrated in the roots than in the stems. The presence of
these specialized metabolites could justify the use of these two organs in the traditional treatment of numerous
pathologies, mainly those linked to oxidative stress.

Keywords: Oxidative stress, phytochemical sorting, UPLC-MS, flavonoids.