European Journal of Biomedical and Pharmaceutical Sciences

AUTOMATED DERVATIZATION WITH O-PTHALDEHYDE FOR THE ASSESSMENT OF AMIN ACIDS IN WOMEN SALIVA USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Alagendran S., Archunan G.*, Anusha Bhaskar and Guevara Guzmán Rosalinda

ABSTRACT

Aim: To find out the ovulation (fertile) specific marker precursor in menstrual cycle phases of women by amino acid profiling during HPLC. Objectives: The current study was carrying out to analysis the feasible changes in salivary amino acids during fertile period. Methods: To detect the ovulatory day we used both cervical/saliva mucus ferning test on a microscope slide to form fern like crystals when it dries which shows the rise and fall of estrogenic and crystals shape which is due to the formation of electrolytes such as sodium and potassium during the spike day (14th day) of LH surge. The present study is expected next to perceptive the existence and amount of amino acids in the saliva of human female at the age of varies reproductive state. High Performance Liquid Chromatography (HPLC) analyses of amino acids during the fertile and infertile period in human saliva followed by OPA photodiode array derivatization followed by FD method. Results: Glutamic acid, Arginine, aspartic acid and GABA are the amino acids uniquely present during the fertile period which revealed the brain noradrenaline precursor, which may cause instability of diverged effect noradrenaline-mediated central functions in susceptible subjects. Further, the amount of GABA and arginine is significantly than that of other amino acids during ovulatory phase (13th day) which posses electric signal followed by electrolyes like sodium and potassium. Conclusion: The method is to assess the clinically important amino acids in saliva may be exceedingly accurate result for fertile period and to avoid the assisted reproductive technology/IVF. Detailed knowledge of the possible variations in human saliva is very important because we can develop a noninvasive problem-solving diagnostic method by assessed the biomarker for amino acids.

Keywords: Amino acids, OPA, estrogen, Saliva, HPLC.