European Journal of Biomedical and Pharmaceutical Sciences

ASSESSMENT OF HISTOCHEMICAL, PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITYOFPTEROCARPUS SANTALINUS L WOOD EXTRACTS

P. Jyothi Chaitanya*, R. Chandrashekar, N. Lakshmi Bhavani

ABSTRACT

Present study focused on the preliminary qualitative histochemical, phytochemical analysis and antimicrobial activity of wood extracts of Pterocarpus santalinus L. It is aimed to find out the Histochemical tests for wood authentication, phytochemical and antimicrobial analysis. Specific tests were conducted to identifyeach group of the phytochemicals of various extracts of wood of Pterocarpus santalinus L. Wood were extracted separately with methanol, ethanol, hot water, petroleum ether and chloroform that were screened for phytochemical constituents. Among all the extracts tested ethanol and methanol extracts showed more phytochemicals than the others followed by petroleum ether, water and chloroform. Analysis revealed the presence of alkaloids, phenols, glycosides, flavonoids, triterpenoids, anthraquinones, steroids and tannins. Ethanol, methanol, petroleum ether, hot water and chloroform wood extracts were tested against selected bacteria and fungi. Ethanol, methanol and petroleum ether showed more inhibition zones against bacteria and fungi than the extracts of chloroform and hot water extracts. The analysis revealed the maximum activity of wood extracts against the Salmonella typhi, E.coli, Streptococci, Bacillus cereus, Pseudomonas aeruginosa and Bacillus subtilis. Klebsiella pneumonia is found to be inhibited by methanol and ethanol extracts only. Extracts of methanol showed broad spectrum inhibition zone against the fungal species Aspergillus Niger, Fusarium, Colletotrichum and Rhizopus than the ethanol, pet ether, chloroform and hot water. The broad spectrum inhibition zone exhibited by Pterocarpus santalinus L may be due to the various active constituents

Keywords: present in it, which either attributed to their individual or combined action exhibit antibacterial activity and antifungal activity. Result indicated that the phytochemical extracts of Pterocarpus santalinus L woodexhibiteddosage dependent antibacterial