ROLE OF CALCIUM ON THE ADSORPTION RATE OF BACTERIOPHAGE AGAINST STAPHYLOCOCCUS AUREUS
Shafeeq-Ur-Rehman, Farhan Rasheed, Ameer Hamza, Taimoor Khan and *Shahid Raza
ABSTRACT
In treating infection caused by staphylococcus aureus which is resistance to antibiotic therapy, Phage therapy
presents an alternative therapeutic option. However it is very much necessary to study the external factors that
may impact the yield and potency of phage preparations proposed for use in various in vitro and in vivo studies.
The present research focuses on the role of calcium on the adsorption rate of a lytic bacteriophage. The presence
of calcium increases the adsorption rate of the phage and also participated in the penetration of the phage genome
in the cytoplasm of the host. During the phage titration process, a final concentration of 1M calcium chloride
supplemented in soft agar, considerably increase the phage titer. However during the isolation of lytic phage
against staphylococcus aureus and during the high titer active phage preparation, incorporation of such divalent
cations would definitely increase the isolation frequency and the final phage yield. This will contribute towards
more effective phage preparation for use in treatment against staphylococcus aureus infection.
Keywords: staphylococcus aureus, cations, infection.
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