CYTOTOXIC AND ANTIMICROBIAL ACTITVITY OF PARTIALLY PURIFIED LECTIN FROM BITTER GOURD SEEDS (MOMORDICA CHARANTIAL.).
Md. Omar Faruk*, Mrityunjoy Biswas, Kamrun Nahar Fatema Dr. M. Shahjahan and Syed Rashel Kabir
ABSTRACT
Lectin was partially purified from Momordica charantia (Bitter gourd) seeds by ion-exchange chromatography on
DEAE cellulose column. The molecular mass of the partially purified Lectin was 115-35 kDa, as estimated by
both in native and denatured SDS-PAGE. The Lectin agglutinated both O+ (ve) human red blood cells as well as
chicken red blood cells. The Lectin Showed poor antibacterial activity against Shigella sonnei and Salmonella
typhi out of nine organisms tested. The partially purified bitter gourd seed Lectins do not show any antifungal
activity against the seven fungi tested. The Dose-mortality test of the partially purified Momordica charantia seed
Lectins was carried out against red flour beetle adults (T. castaneum) throughresidual film bioassay method. The
result showed promising insecticidal activity against these beetles with LD50 values of 40.43 to 23.61 mg/cm2
after 12 to 48 hrs of exposure. The Lectin showed slight toxicity to brine shrimp nauplii, the calculated LC50
value was 259.5. In vivo cytotoxicity study of this Lectin in Swiss albino mice showed a moderate toxic effect.
Keywords: Antibacterial, Chromatography, Cytotoxicity, Bioassay.
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