European Journal of Biomedical and Pharmaceutical Sciences

MOLECULAR DETECTION OF SHV GENE PRODUCING KLEBSIELLA SPECIES IN STOOL SAMPLES COLLECTED FROM A TERTIARY HEALTH FACILITY

Pughikumo D. T.*, Alade T., Eromosele P. O. and Pughikumo O. C.

ABSTRACT

Bacterial resistance to antibiotics has been a major source of public health concern. Klebsiella pneumonia an important health pathogen is highly resistant to a wide range of antimicrobials and antibiotics. Investigation of beta-lactamase (extended-spectrum) - ESBL encoding-resistant gene, SHV in Klebsiella pneumonia isolated from clinical samples at a tertiary health facility, using standard Bacteriological technique. From fifty samples of clinical isolates used, thirty-three (33) were positive in this study for Klebsiella pneumonia, E. coli, Proteus mirabilis. 17 isolates were from female and 16 from males. 10 (100%) of the 33 samples were Klebsiella pneumonia isolates. Klebsiella pneumonia showed indole negative, citrate positive, it does not ferment lactose but ferment glucose, does not produce hydrogen sulphide but produces gas on Kliger Iron Agar (KIA). Amplification of SHV gene was done using PCR (Polymerase Chain Reaction) technique. The age base profiling of bacterial isolates showed that Klebsiella pneumonia was highest (80%) among 11 to 30 years. Antimicrobial susceptibility test was performed using disk diffusion method. The susceptibility pattern in the study revealed that Klebsiella pneumonia exhibited the highest susceptibility of 7(70%) for Reflacine, Streptomycin, Tarivid. 6 (60%%) for Ciprofloxacin and Gentamycin, and moderate susceptibility of 4(40%) for Sceptrin, 3(30%) Amplicin, Augmentin, and Nalidixic Acid. 2(20%) for Ceporex, which showed a high resistance to Klebsiella pneumonia. Identification of the gene that encodes plasmid transfer of SHV gene was done using multiplex PCR technique. From the results, accurate identification of resistant Gram-negative bacteria such as Klebsiella pneumonia and detection of its susceptibility to common antibiotics might enhance treatment and control of resistant nosocomial infections causing Klebsiella pneumoniae.

Keywords: Klebsiella pneumonia, SHV, antibiotics, resistant gene, ESBL.