European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION AND SCREENING OF LACTOBACILLUS SP. KLSA 22 FOR LACTASE PRODUCTION UNDER SUBMERGED FERMENTATION

Sarfaraz Ahmed, Mahesh Divatar, Sandhya Gajare, Shivalee A and Lingappa Kattimani*

ABSTRACT

Lactase is one of the commercially important enzymes produced by wide range of microbes. The aim of the present study was to isolate and screen the lactic acid bacteria from different samples collected from milk processing areas and dairy effluents for lactase production. In this attempt a total of sixty five lactic acid bacteria were isolated and screened for lactose utilization. Further, these isolates were subjected for lactase productions under Submerged Fermentation (SmF) using DeMan Rogosa and Sharpe (MRS) broth. In lactase assay, onitrophenyl- β-D-galactopyranoside (ONPG) was used as a chromogenic substrate to determine the lactase activity of bacterial isolates. The isolates KLSA 16 KLSA 22 KLSA 27 KLSA 34 and KLSA 62 were found with good lactase activity. Finally the isolate KLSA 22 was selected for further study as it shown comparatively highest yield of lactase. The highest lactase activity achieved under described optimum conditions by KLSA 22 was 89.77 IU.

Keywords: LAB, ?-galactosidase, Lactose utilization, Cheese Whey, ONP.