OPTIMIZATION OF Alpha-AMYLASE PRODUCTION BY USING SOLID STATE FERMENTATION TECHNOLOGY FROM Bacillus subtilis SSD-I (JQ747516) IN LONAR LAKE [MS] INDIA
*Sudarshan S. Pedge and Sunil D. Ahirrao
ABSTRACT
Microbial Alpha amylase is highly demanded industrial enzyme in
various sectors such as pharmaceuticals, food, textile and detergents,
etc. alkaline bacterial stains has better ability to produce such type
enzyme. In the present study, alkaline bacterial strains were isolated
from water samples collected from saline Lonar Meteorite crater, (MS) India having pH 10.5.
Among them one of the bacterial strains was identified by 16S rRNA sequencing showed
95% alignment match with Bacillus subtilis. The optimum growth of such organism was 12.5
pH and 16% salt concentrations. Chemical analysis of water shows metal ions and salt
concentrations in Lonar crater. The production of α-amylase by producing Bacillus subtilis
JQ747516 was studied under solid state fermentation (SSF). Different agro-based products as
substrates were studied for enzyme production. Production parameters were optimized as
incubation time 72 hrs, incubation temperature 37oC, initial moisture 30%, and initial pH 10.
Supplementation of the fermentation medium with carbon and metal ion sources decreased α-
enzyme production. Among different nitrogen sources supplemented, ammonium nitrate
(1%) showed maximum amylase production (1470.3 ± 3.56 U g-1.10-3).
Keywords: 16S rRNA, alpha-enzyme, SSF, Lonar Lake.
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