Abstract
OPTIMIZATION OF Alpha-AMYLASE PRODUCTION BY USING SOLID STATE FERMENTATION TECHNOLOGY FROM Bacillus subtilis SSD-I (JQ747516) IN LONAR LAKE [MS] INDIA

*Sudarshan S. Pedge and Sunil D. Ahirrao

ABSTRACT

Microbial Alpha amylase is highly demanded industrial enzyme in various sectors such as pharmaceuticals, food, textile and detergents, etc. alkaline bacterial stains has better ability to produce such type enzyme. In the present study, alkaline bacterial strains were isolated from water samples collected from saline Lonar Meteorite crater, (MS) India having pH 10.5. Among them one of the bacterial strains was identified by 16S rRNA sequencing showed 95% alignment match with Bacillus subtilis. The optimum growth of such organism was 12.5 pH and 16% salt concentrations. Chemical analysis of water shows metal ions and salt concentrations in Lonar crater. The production of α-amylase by producing Bacillus subtilis JQ747516 was studied under solid state fermentation (SSF). Different agro-based products as substrates were studied for enzyme production. Production parameters were optimized as incubation time 72 hrs, incubation temperature 37oC, initial moisture 30%, and initial pH 10. Supplementation of the fermentation medium with carbon and metal ion sources decreased α- enzyme production. Among different nitrogen sources supplemented, ammonium nitrate (1%) showed maximum amylase production (1470.3 ± 3.56 U g-1.10-3).

Keywords: 16S rRNA, alpha-enzyme, SSF, Lonar Lake.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT