European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTI-UROLITHIATIC ACTIVITY OF MIXTURE OF TRIDAX PERCUMBENES & KALANCHOE PINNATA EXTRACT BY UV SPECTROSCOPY

Anuja Nanasaheb Nikam*

ABSTRACT

In medical terminology, kidney stone disease is referred to as renal calculus disease, nephrolithiasis, or urolithiasis. The Latin word "renal" means kidney, and the Greek word "nephro" means kidney. Both "calculus" (Lat.-pl. calculi) and "lithiasis" refer to stone. A crystallopathy known as kidney stone disease is brought on by an excess of minerals in the urine combined with dehydration. Small crystal fragments collect in the upper urinary system as calculi, or stones, as a result of this imbalance. If renal calculi are tiny enough, they can exit the urinary tract through the urine stream because they usually develop in the kidney. A little calculus could go away without producing any symptoms. But, if a stone becomes larger than 5 millimeters (0.2 inches), it may obstruct the ureter, which can lead to renal colic, a strong, excruciating pain in the lower back that frequently travels down to the groin.[1] The majority of calcium stone patients (75%–80%) have nephrolithiasis, which is mainly caused by calcium oxalate monohydrate or dehydration. Hypercalciuria, hyperoxaluria, hyperuricosuria, and hyppcitraturia are the four most prevalent chemical factors that contribute to the production of urinary stones.[2] The primary need and goals are: Preventing kidney stones is the main goal of anti-urolithiatic activity. Inhibiting crystal formation stops them from growing and aggregating. To avoid kidney stone-related urinary diseases. Materials and Procedures: Fresh Kalanchoe pinnata leaves were extracted in water and arranged in various concentrations. Artificial stones like calcium phosphate and calcium oxalate were made using the homogenous precipitation method, and semi-permeable egg membranes were utilized as dissolution bags. Following a 72-hour incubation period, spectrophotometric estimation was used to determine the total content of the dissolving bags. The weight variation test of calcium oxalate tablets was used to assess the inhibitory effect of a combination of Kalanchoe pinnata and tridax percubenes extract on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals. Findings: While the Cystone standard has demonstrated stronger demineralization for calcium phosphate than the extract of Kalanchoe pinnata, the extract of a combination of Kalanchoe pinnata and tridax percubenes had a greater capacity to dissolve calcium oxalate in dissolving models. In the nucleation assay as opposed to the aggregation assay, cysteine demonstrated a considerable inhibitory effect. Tridax percubenes extract and Kalanchoe pinnata extract together had a strong inhibitory effect in both the nucleation and aggregation tests. Discussion: The current study will contribute to the body of knowledge regarding in-vitro research. Understanding the molecular mechanism of the litholysis process and identifying the phytochemicals in the extract that dissolve or disintegrate renal calculi may be aided by correlation between in vitro investigations. Conclusion: Tridax percubenes extract and Kalanchoe pinnata extract together demonstrated strong in-vitro anti-urolithiatic activity.

Keywords: Kalanchoe Pinnata, Tridax Percumbenes, Spectrophotometer, Semipermeable membranes.