EVALUATION OF ANTI-NOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY OF NYMPHAE ALBA FLOWER EXTRACT
Ramji Kushwaha*, Manju Prajapati and Akhlesh Kumar Singhai
ABSTRACT
The objective of the study was to assess the anti-nociceptive and anti-inflammatory potential of the 80% methanolic extract of Nymphae alba flowers. The extract was sticky, black in color with a yield of 16.8%. The preliminary phytochemical analysis suggest the presence of phenolics and tannins, proteins and flavonoids in the 80% methanolic extract of the flowers. The total phenolic content of the 80% methanolic extract of Nymphae alba was 51.72 ± 1.418 GAE mg/g. The total flavonoid content of 80% methanolic extract of Nymphae alba was found to be 46.42 QE mg/g. The anti-inflammatory activity was assessed using carrageenan induced rat paw edema method while the analgesic action was determined using tail flick method. Ibuprofen at dose of 10 mg/Kg inhibited 76.76% edema after 6h of administration while the NAE 200 and NAE 400 were able to inhibit 46.13% and 52.68% edema formation respectively. The duration of response time in Ibuprofen and Nymphae alba was significantly higher as compared to the saline treated animals. The reaction time for NAE 200 and NAE 400 was 6.092 ± 0.117 sec and 6.596 ± 0.203 sec respectively at 60 min post administration while it was 3.396 ± 0.045 sec and 7.994 ± 0.287 sec for vehicle treated group and Ibuprofen respectively at the same time duration.
Keywords: Nymphae alba, Anti-nociceptive, Anti-inflammatory, Methanolic extract.
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