FORMULATION AND EVALUATION OF SOLID LIPID NANOPARTICLES OF CASSIA ALATA EXTRACT FOR MANAGEMENT OF WOUND
Mohit Khare, *Vinod K Dhote, Kanika Dhote and Surendra Jain
ABSTRACT
The objective of the present research was to investigate the wound-healing potency of solid lipid nano particles of cassia alataextract. Preliminary phytochemical screening revealed the presence of various active constituents in the methanolic extract, including alkaloids, glycosides, carbohydrates, flavonoids, and tannins, which are known for their pharmacological benefits. These findings justified the use of the methanolic extract for SLN formulation. The methanolic extract demonstrated a higher percentage yield and richer phytochemical profile compared to petroleum ether extract, making it more suitable for nanoparticle preparation. The extraction process using methanol and petroleum ether yielded 2.26% and 0.786% respectively, confirming methanol as a more efficient solvent for extracting bioactive compounds. Among the five formulations, SLN3 and SLN4 exhibited desirable particle size and PDI values, essential for effective drug delivery. SLN4, with the smallest particle size (97.23 nm), and SLN3, with the lowest PDI (0.212), showed excellent potential in terms of particle uniformity and bioavailability. Particle size analysis showed that SLN4 had the smallest particle size (97.23 nm), followed by SLN3 (142.51 nm), with SLN3 having the lowest polydispersity index (PDI = 0.212), indicating high uniformity and stability. Zeta potential values ranged from -8.7 mV to 19.4 mV, suggesting acceptable colloidal stability across all formulations. In conclusion, the optimized formulations, particularly SLN3 and SLN4, hold potential for further in vivo studies and future development into stable, effective herbal-based nanomedicines for treating infections, inflammation, and related conditions.
Keywords: Solid lipid nanoparticles, cassia alata, Wound healing, Antioxidant, Zeta potential, Soxhlet extraction, Phytochemical analysis.
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