European Journal of Biomedical and Pharmaceutical Sciences

EFFECT OF OXYTOCIN AND BROMOCRIPTINE INJECTION ON THE DURATION OF PSEUDOPREGNANCY AND THE LEVELES OF PROLACTIN, LUTEINIZING AND PROGESTERONE HORMONES IN PSEUDOPREGNANT RATS

Ahmed Aboud Khalifa*, Adnan Salih Al-Janabi and Falih Hassan Al-Diwan

ABSTRACT

The present study was conducted to investigate the effect of oxytocin (OT) and Bromocriptine (BR) on duration of pseudopregnancy (pp) and the levels of prolactin (Prl), luteinizing (LH) and progesterone (P) hormones in pseudopregnant rats. OT was injected twice daily subcutaneously to pseudopregnant rats. This study involved two experiments; the first was designed to study duration of pseudopregnancy before and after treatment with: OT 500 and 600 m. u; OT+Bromocriptine (BR) (500m. u. +0. 5mg) and BR (0. 5mg) alone, at day 6 of pp for 4 days. The second experiment included treatment with the same substances and doses (except OT 600 m. u.) at day 6 of pp and scarified on day 7 (Group 1),day 8 (Group 2) and on day 11 and 12 (Group 3) which was treated with OT only. Results revealed: a significant (P < 0. 01) increase and decrease in the length of the pp due to injection of OT (500m. u. and BR alone respectively. Hormonal changes revealed mostly a significant (P < 0. 01) increase in prolactin and progesterone serum levels in OT treated rats, however, a significant (P < 0. 01) reduction was seen in other treatments. LH levels showed a tendency for a reduction which was significant (P< 0. 01) in most animals of group 1 and 2. However, a significant (P < 0. 01) increase in LH concentration in OT treated animals of group 3. These results may indicate that OT has a stimulatory effect on rat s corpora luteal activity i. e. it’s a luteotropic. This activity is mediated probably through stimulation of prolactin release from the anterior pituitary gland.

Keywords: Oxytocin, Prolactin, Bromocriptin, Pseudo pregnancy, Luteinizing hormone, Progesterone. This research is apart of PhD.thesis for the first auther.