EVALUATION OF NOOTROPIC ACTION OF METHANOLIC FRACTION FROM ETHYLACETATE EXTRACT OF GREWIA HIRSUTA BARK
Swadha Kumari Sah*, Ashish Chaurasia
ABSTRACT
The objective of the present work was to prepare the ethylacetate extract of bark of Grewia hirsuta and obtain the methanolic fraction of the extract and evaluate its nootropic potential in rodent. The extraction yield of the bark of Grewia hirsuta in methanol was found to be 17.47% w/w. The preliminary phytochemical analysis suggest the presence of phenolics, tannins, flavonoids, protein, sterol and terpenes in the ethylacetate extract. The ethylacetate extract of Grewia hirsuta was quantified for the total phenolic content. The total phenolic content of ethylacetate fraction was found to be 154.87 ± 1.416 GAE mg/g. The extract was fractionated with methanol using a separating funnel and the methanolic fraction was test found to test positive for flavonoids and phenolics. The methanolic fraction was evaporated to obtain dark yellow powder in 21.6 % w/w yield. The spectral analysis of the methanolic fraction revealed the presence of three components in UV spectra (λmax 305.0, 261.0 and 224.0 nm). The FTIR spectra suggested hydroxyl, amine, carbonyl and aromatic functionalities in the fraction. The methanolic fraction of the ethylacetate extract of Grewia hirsuta bark was subjected to evaluation of nootropic potential using morris water maze test at dose levels of 200 and 400 mg/kg/p.o against scopolamine induced amnesia. The mice treated with 400 mg/kg MFGH required only 15.50 ± 1.378 seconds on the 4th day to find the hidden platform as compared to 50.83 ± 2.228 for the scopolamine treated mice.
Keywords: Grewia hirsuta, nootropic, scopolamine, flavonoids, fractionation.
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