ANTIINFLAMMATORY AND ANTIOXIDANT ACTIVITY OF POMEGRANATE PEEL EXTRACT
B. L. Nanda*, B. S. Vishwanath
ABSTRACT
Secretory PLA2 (sPLA2-IIA) is implicated in inflammatory responses because a high level of this enzyme has been
found in inflammatory fluids and cells. PLA2 enzyme catalyses the breakdown of membrane phospholipid to
arachidonic acid and lysophospholipid. Arachidonic acid is in turn converted to proinflammatory mediators and
ROS. A single molecule having both antioxidant and PLA2 inhibition is of great therapeutic importance in
designing anti-inflammatory molecules. Pomegranate peel extracted with different solvents with increasing
polarity were screened for phenolics, antioxidant activity and sPLA2 inhibition. The acetone extract exhibited high
antioxidant activity and sPLA2 inhibition. The concentration of phenolics directly correlated with antioxidant
activity and PLA2 inhibition. P.granatum peel acetone extract also showed dose dependent inhibition of sPLA2
enzyme both by invitro and invivo method. The P.granatum peel acetone extract was further subjected to TLC for
separation of components in extract. The extract showed 7-8 bands. Each band was subjected to PLA2 inhibition
and antioxidant activity. PPCII was found to have high PLA2 inhibition and antioxidant activity. The extract may
contain compounds (Phenolics, anthocyanins, flavonoids, tannins, alkaloids) which are both antioxidant and antiinflammatory
in nature and they can be used for clinical development as a new class of anti-inflammatory agents
with antioxidant activity.
Keywords: Anti inflammatory, Antioxidant, Free radicals, Phenolics, secretory phospholipase A2, Punica granatum.
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