IDENTIFICATION OF NOVEL BIOMARKER FOR THE MEDICINALLY IMPORTANT SPECEIS OF SELAGINELLA USING MALDI TOF MS ANALYSIS
Narayani Mani*, Johnson Marimuthu Alias Antonysamy and Sakthivel Ramasamy
ABSTRACT
MALDI profiling has become a good tool for the discovery and validation of biomarkers. In the present study,
MALDI-TOF MS has been applied as a tool to identify the biochemical (protein) similarities and variations among
the selected Selaginella species viz., Selaginella intermedia (Bl.) Spring, Selaginella inaequalifolia (Hook. et
Grev.) Spring, Selaginella involvens (Sw.) Spring, Selaginella tenera (Hook & Grev.) Spring, Selaginella wightii
Hieron., Selaginella brachystachya (Hook. & Grev.) Spring, Selaginella repanda (Desv.) Spring, Selaginella
radicata (Hook. & Grev.) Spring, Selaginella bryopteris (L.) Bak and Selaginella delicatula (Desv.) Alston from
their natural habitats of South India. MALDI-TOF MS characterization of Selaginella species collected from
various localities of South India expressed different ion peaks ranged from 0 - 1, 00,000 kDa. A total of 556
ranging from 491 to 99939 m/z values MALDI-TOF MS spectral peaks were observed. Among the spectral profile
of ten Selaginella species, S. involvens displayed maximum number (84) of spectral peaks ranged from 527 to
99215 m/z values and S. radicata depicted minimum number (21) of spectral peaks varied from 694 to 91754 m/z
values. Totally, 139 spectral peaks showed the inter-species resemblance among the studied medicinally important
Selaginella species. The cladogram constructed based on the unique spectral values displayed both monophyletic
group and paraphyletic taxon. The distinguished character of S. tenera, S. involvens, S. wightii, S. brachystachya,
S. repanda and S. delicatula is confirmed in the cladogram by the presence of unique m/z values. The unique m/z
values can be act as a biochemical marker to identify the specific Selaginella species in chemosystematics and
pharmaceutical industries.
Keywords: MALDI-TOF MS, Selaginella, monophyletic, paraphyletic, chemosystematics.
[Full Text Article]
[Download Certificate]
Impact Factor : 8.181 
