PHARMACOLOGICAL EVALUATION, ANTIOXIDANT AND ANTI UROLITHIC ACTIVITY OF SINAPIS ARVENSIS LEAVES EXTRACT IN EXPERIMENTAL RATS
Priyanka Sharma *, Dr. Abhishek Sharma, Ms. Payal Saiju, Dr. Surendra Jain
ABSTRACT
The present study investigates the pharmacological, antioxidant, and anti
urolithic poten tial of methanolic leaf
extract of Sinapis arvensis in experimental rats. Methanol extraction yielded 22.45 g from 500 g of dried plant
material, with an e xtraction efficiency of 11.55%. Phytochemical screening revealed the presence of alkaloids,
glycosides, carbohydrates, flavonoids, phenolics, proteins, and saponins, which are known to contribute to
therapeutic activity. Total phenolic and flavonoid conten t were measured as 68.3 mg/g and 53.9 mg/g gallic acid
and rutin equivalents, respectively. Antioxidant activity assessed by DPPH assay demonstrated moderate radical
scavenging activity with an IC ₅₀ of 48.46 μg/mL, compared to 21.40 μg/mL for ascorbic acid. FTIR analysis
confirmed the presence of functional groups related to bioactive constituents. In the ethylene glycol induced
urolithiasis model, the extract, particularly at 400 mg/kg, significan tly reduced elevated serum creatinine, urea,
calcium, and phosphorus levels, and indicating anti urolithic effects. These findings support the traditional use of
Sinapis arvensis and suggest its potential as a natural therapeutic agent for managing urolith iasis and oxidative
stress.
Keywords: Sinapis arvensis, Methanolic extract, Antioxidant activity, DPPH assay, Urolithiasis, Phytochemicals, FTIR analysis.
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