EVALUATING THE GENETIC AFFINITY OF SELAGINELLA SPECIES USING DIFFERENT ISOZYMIC PROFILES
Narayani Mani*, Johnson Marimuthu Alias Antonysamy and Sakthivel Ramasamy
ABSTRACT
The present study was intended to identify the genetic variability among the selected species of Selaginella viz.,
Selaginella intermedia (Bl.) Spring, Selaginella inaequalifolia (Hook. et Grev.) Spring, Selaginella involvens (Sw.)
Spring & Selaginella tenera (Hook & Grev.) Spring), Selaginella wightii Hieron., Selaginella brachystachya
(Hook. & Grev.) Spring & Selaginella repanda (Desv.) Spring, Selaginella radicata (Hook. & Grev.) Spring,
Selaginella bryopteris (L.) Bak, Selaginella delicatula (Desv.) Alston with cones), Selaginella delicatula (Desv.)
Alston without cones were collected from Western Ghats, South India using different isozymic profiles. PAGE
was carried out using standard procedure of Sadasivam and Manickam (1991). The isoenzymes selected for the
study includes, esterase, peroxidase, acid phosphatase, alkaline phosphatase and polyphenol oxidase. Multiple
regions of activity with a total of thirty eight esterase bands, sixty six peroxidase bands, sixty three acid
phosphatase bands, forty eight alkaline phosphatase bands and twelve polyphenol oxidase bands were observed in
the isozymic system of studied Selaginella species. Unique banding profiles of esterase, peroxidase, acid
phosphatase, alkaline phosphatase and polyphenol oxidase which observed in the studied Selaginella species were
used to distinguish the selected Selaginella species and these isozymic profiles will act as biochemical fingerprint
for the studied species in plant systematic studies.
Keywords: Selaginella; Isozymic profiles; PAGE; Biochemical fingerprint; Plant systematic.
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