PHARMACOLOGICAL EVALUATION OF IPOMOEA TRILOBA LEAF EXTRACT IN WISTAR RATS
Preeti Soni*, Ankit Mehra, Ravi Prakash
ABSTRACT
The present study was undertaken to evaluate the antioxidant and anti-ulcer potential of Ipomoea triloba leaf
extract using in vitro and in vivo experimental models. Phytochemical screening of the methanolic extract
revealed the presence of bioactive constituents such as phenolics, flavonoids, glycosides, tannins, and terpenoids.
Quantitative analysis showed high total phenolic content (52.5 mg gallic acid equivalent/g extract) and total
flavonoid content (27.5 mg rutin equivalent/g extract), indicating a rich antioxidant profile. The in vitro
antioxidant activity assessed by the DPPH radical scavenging assay demonstrated significant free radical
scavenging potential with an IC₅₀ value of 48.61 μg/mL. Acute oral toxicity studies conducted as per OECD-423
guidelines showed no signs of toxicity or mortality up to a dose of 2000 mg/kg, confirming the safety of the
extract. In the indomethacin-induced gastric ulcer model in Wistar rats, Ipomoea triloba extract exhibited dosedependent
gastroprotective effects. The higher dose (400 mg/kg) significantly reduced ulcer index, gastric juice
volume, and free acidity while improving gastric pH, with effects comparable to the standard drug ranitidine.
Macroscopic examination of gastric tissues supported the biochemical findings, showing reduced mucosal damage
and enhanced tissue protection. These results validate the traditional use of Ipomoea triloba and suggest its
potential as a safe, natural anti-ulcer agent, warranting further mechanistic and clinical investigations.
Keywords: Ipomoea triloba; Anti-ulcer activity; Antioxidant activity; Phytochemical analysis; Indomethacininduced gastric ulcer; Wistar rats.
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