ASSESSMENT OF PERCENTAGE RECOVERY STUDY OF TETRABENAZINE AND ITS PHARMACEUTICAL DOSAGE FORMS BY UV
Monika Sahu*, Ankit Mehra, Ravi Prakash
ABSTRACT
Spectroscopy is a fundamental analytical technique that examines the interaction between electromagnetic
radiation and matter and is extensively applied in pharmaceutical analysis for both qualitative and quantitative
purposes. Among various spectroscopic techniques, ultraviolet–visible (UV–Vis) spectroscopy is widely
employed due to its simplicity, rapid analysis, accuracy, and cost-effectiveness. The present study was aimed at
the development and validation of a simple, precise, and economical UV spectrophotometric method for the
quantitative estimation and percentage recovery assessment of tetrabenazine in pharmaceutical dosage forms.
Tetrabenazine, a vesicular monoamine transporter-2 (VMAT2) inhibitor used in the treatment of chorea associated
with Huntington’s disease, requires accurate dosage determination due to its narrow therapeutic index. Preformulation
studies were carried out to evaluate the physicochemical properties of the drug, including organoleptic
characteristics, melting point, solubility, pH, and compatibility. Tetrabenazine exhibited good solubility in ethanol,
which was selected as the analytical solvent. UV spectroscopic analysis revealed a maximum absorbance (λmax)
at 350 nm. The method demonstrated excellent linearity in the concentration range of 20–120 μg/mL with a
correlation coefficient (R²) of 0.9999, confirming compliance with Beer–Lambert’s law. The developed method
was validated in accordance with ICH guidelines for specificity, linearity, accuracy, precision, ruggedness,
robustness, limit of detection, and limit of quantitation. The percentage recovery of approximately 98% indicated
good accuracy, while %RSD values below 2% confirmed precision and reproducibility. The validated UV
spectrophotometric method is reliable, rapid, and suitable for routine quality control analysis of tetrabenazine in
bulk drug and tablet dosage forms.
Keywords: UV–Visible spectroscopy; Tetrabenazine; Method validation; Pharmaceutical analysis; Beer– Lambert law; Quality control.
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