INVESTIGATION OF THE RENO PROTECTIVE EFFECTS OF HERBAL PLANT EXTRACT ON EXPERIMENTALLY INDUCED NEPHROTOXICITY IN RATS
Sudhir Chaudhary*, Praveen Kumar Jain, Ankit Mehra
ABSTRACT
The present study investigated the nephroprotective potential of Golden leather fern, a traditionally used
mangrove fern with reported medicinal properties. The plant was selected based on ethnobotanical evidence
supporting its role in renal and oxidative stress–related disorders. Fresh plant material was collected,
authenticated, shade-dried, powdered, and subjected to Soxhlet extraction to obtain bioactive constituents. The
percentage yield was calculated to assess extraction efficiency. Preliminary phytochemical screening was
performed to identify major classes of bioactive compounds, and quantitative estimation of total phenolic and
flavonoid contents was carried out using standard colorimetric assays. The antioxidant activity of the extract was
evaluated using the DPPH free radical scavenging assay. Acute oral toxicity studies were conducted according to
OECD guidelines to establish the safety profile and determine appropriate dose levels. Nephroprotective activity
was assessed in albino rats with experimentally induced nephrotoxicity. Renal function was evaluated by
measuring urine output, body weight changes, serum creatinine, and blood urea nitrogen levels. Histopathological
examination of kidney tissues was performed to assess structural and cellular alterations. Statistical analysis of the
results demonstrated a significant nephroprotective effect of the plant extract, likely mediated through its
antioxidant and phytochemical constituents. The findings support the traditional use of Golden leather fern and
suggest its potential as a natural therapeutic agent for the management of renal disorders.
Keywords: Nephroprotection; Golden leather fern; Antioxidant activity; Phytochemical screening; Renal toxicity; Albino rats; Oxidative stress.
[Full Text Article]
[Download Certificate]
Impact Factor : 8.181 
