European Journal of Biomedical and Pharmaceutical Sciences

STUDY OF ANTIMICROBIAL ACTIVITY OF HIBISCUS SABDARIFFA LEAF EXTRACT USING IN-VITR DISK DIFFUSION METHOD

Farhanajmi Khan, Wasim Raja*

ABSTRACT

Hibiscus sabdariffa L. is widely used in the preparation of beverages. Active component of this plant exhibit antimicrobial activity, yet little research has been conducted on their possible use in food systems as antimicrobials. The use of this tradicinal medicinal plant as natural antimicrobial agents is gaining popularity. The Hibiscus sabdariffa is widely used for the treatment of diseases. The present study is the continuation of a program aimed at investigation of antimicrobial properties of Hibiscus sabdariffa leaf extract. The antibacterial activity was evaluated according to the disk diffusion method by using Gram positive; B. subtilis, S. aureus and Enterococcus and Gram negative; E. coli, Acetobacter, citrobacter, bacteria. This study shows that methenolic leaves extracts of Hibiscus sabdariffa Linn inhibit the growth of microorganism’s dose dependently. The appear results confirm that the antibacterial activity of Hibiscus sabdariffa leaves.

Keywords: Antibacterial. Disk Diffusion method. Bacteria. Hibiscus sabdariffa.