European Journal of Biomedical and Pharmaceutical Sciences

ANTIPYRETIC EFFECTS OF EXTRACTS AND SOLVENT FRACTIONS FROM TRICLISIA GILLETII (DE WILD) STANER (MENISPEMACEAE) ROOT BARK TOWARDS BREWER’S YEAST INDUCED PYREXIA IN SUISS ALBINO MICE

Mangombo K. H., Kikweta M. C., Mampuya M. C., Lusakibanza M., Mesia K. G., Tona L. G., Cimanga K. R.*

ABSTRACT

The present study to the investigation of aqueous and it solvent fractions as well as methanol 80%  from Triglisia gilletii rotr barks by using the toxican Brewer’s yeast induced high rectal temperatures in Swiss albino mice was conducted. The difference between 0 hour and respective time intervals of recorded temperatures compared to toxic negative control Brewer’s yeast group was performed. Results revealed that the subcutaneous injection of the toxican Brewer’s yeast induced high rectal temperatures from 39.9±0.09 to 40.3±0.5°C in 1 and after 5h of administration the toxican. Next, the oral administration of Aspirin as reference antipyretic drug at dose of 100 mg/kg bodyweight in mice with pyrexia reached significant lowering of rectal temperatures from 37.5±0.2 to 34.6±0.8 after 1 and 5h of treatment. In the same manner, the oral administration of aqueous and methanol 80% extracts at high dose of 400 mg/kg bodyweight caused remarkably decrease of rectal temperature to values of 38.0±0.6 and 37.5±0.5°C in 1h of treatment and continued to land until to 37.0±0.4 and36.5±0.5°C after 5h of treatment. Moreover, the oral administration of chloroform, ethylacetate, n-butanol and residual aqueous solvent fractions at the same oral dose reached also significant lowering of rectal temperatures from 37.3±0.5 to 39.0±0.6°C  and from 37.0±0.5 to 36.7±0.5 after 1  and 5h of treatment respectively while crude polysaccharides acted the same manner by producing significant reduction of rectal temperatures from 38.7±0.7 to 36.5±0.8°C after the same times of treatment. In definitive, these significant lowering of rectal temperatures in treated mice with pyrexia compared to toxic Brewer’s yeast clearly demonstrated that these samples from Triglisia gilletii root bark were endowed with good and interesting antipyretic effects in animal model.

Keywords: Triclisia gilletii, Menispermaceae, root barks, extracts, solvent fractions, crude polysaccharide, antipyretic activity.