European Journal of Biomedical and Pharmaceutical Sciences

POTENTIAL ANTIOXIDANT ACTIVITY OF THE EXTRACTS AND THEIR ACTIVE COMPONENTS OF PHYSALISPERUVIANA L. (CAPE GOOSEBERRY) AND PHYLLANTHUS (AMALA)

*Anil Kumar

ABSTRACT

The extraction of secondary metabolites was carried out by using water, a mix of methanol and water (8:2) with
NaF, as well as methanol, ethanol, and acetone (all mixed with water in a 7:3 ratio) from various parts (like leaves,
flowers, stems, and roots) of Physalisperuviana L.(Cape Gooseberry) and Phyllanthus emblicand (Amala) with
the help of decoction and maceration methods. This paper states that methanol provides the best results for
decoction, while acetone worked best for maceration. The total polyphenol content (TPC) achieved through
decoction showed the highest TPC levels, and MeOH with NaF was found to be the most effective solution for
extracting TPC. Maceration proved better for extracting flavonoids, with ethanol and acetone being the most
effective solvents. In general, the highest levels of TPC and flavonoids were obtained from leaves and fruits of
Physalisperuviana L. (Cape Gooseberry) and Phyllanthus (Amala) regardless of the solvent or extraction method
applied. Furthermore, the roots of Physalisperuviana L.(Cape Gooseberry) and Phyllanthus emblicand (Amala)
showed important levels of these compounds in consonance with the total antioxidant activity (TAA) evaluated in
the different organs of the plant in the these species. In this study, the solvents and extraction methods applied
were tools that determined significantly the level of extraction of bioactive compounds, showing a different impact
on plant organs for each medicinal species studied.

Keywords: maceration, decoction, TPC, flavonoids, bioactive molecule, antioxidant activity.