COMPARISON OF CURCUMIN CONTENT OF SOME TURMERIC SAMPLES COLLECTED FROM DIFFERENT PLACES OF NORTHEAST INDIA
Rimi Paul, Barnali Gogoi, K. Zaman and H. K. Sharma*
ABSTRACT
Curcumin, derived from the rhizome Curcuma longa, is one of the primary ingredients in Turmeric .Curcumin, the
active molecule present in Curcuma longa is known for its antitumour, antioxidant, antiarthritic, antiinflammatory
and antimalarial activities. The pharmacological activity of Curcuma longa depends on quality of
curcumin present. Turmeric samples were collected from different places of Northeast India and analyzed for its
curcumin content and genetic diversity with RAPD technique. The results of the study disclose that in Hydroalcoholic
extract, turmeric samples collected from Shillong and Arunachal Pradesh have comparatively higher
percentage of curcumin than the other samples. In case of Methanolic extract turmeric samples of Shillong and
Dibrugarh have higher percentage of curcumin as compared to the other samples. The HPTLC separation was
performed on precoated aluminium backed HPTLC plates of 0.2 mm layer thickness with silica gel 60 F254
with Chloroform : Methanol : Acetic acid (10:1:2) combination as mobile phase. The plate was developed up to
80 mm at temperature of 200C with 10 min. of chamber saturation. Under this condition the retardation factor
(Rf) of curcumin is 0.17, 0.42 and the compound was quantified at its absorbance maxima (λmax) at 366 nm
nm.The proposed method is useful as an accurate, simple, cost effective and sensitive for quantitative estimation
of curcumin. The findings reveal the curcumin content depends on geographical variation which influences the
soil, environment, climatic conditions etc.
Keywords: Curcuma longa, Curcumin, HPTLC, Spectrophotometric, Geographical distribution.
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