SEPARATION AND PARTIALLY PURIFICATION OF AN ANTIFUNGAL PROTEIN ON AGAROSE GEL ELECTROPHORESIS FROM PEARL MILLET SEEDS
Ojasvi S. Zadokar, Sangita S. Kunjwani, Z. H. Khan* and Nazia D. Khan
ABSTRACT
One of main challenges’ faced by growing crops successfully, comes from the natural enemies of plants including
bacterial, viral and fungal pathogens .Antifungal proteins (AFPs) are ubiquitous components in different plant
parts, belong to a group of plant defense protein that have a broad spectrum of biological activity and play a key
role in plant defense against pathogenic fungi by preventing or limiting their spread. Occurrence of AFPs in seeds
might be involved in protecting the seeds during resting or storage germination. Various antifungal protein have
been studied from different plant seeds, that help to protect the seeds from the invading soil-borne plant pathogens.
The main objective of proposed study was to isolate and partial purification of antifungal protein from Pearl millet
seeds and its analysis on agarose gel electrophoresis .Pearl millet is known by the name of scientific name of
“Pennisetum glaucum. The protein was isolated by 60% ammonium sulphatesalt fractionation method and
partially purified by dialyzing the sample against sodium phosphate buffer ( pH=7).The protein was analysed on
1% agarose gel electrophoresis. Single band was obtained corresponding to the molecular mass of
25KDa.Antifungal activity against Aspergillusniger was tested which had shown the inhibition of A.niger after the
plates were incubated for 48 hours.
Keywords: Pearl millet, antifungal protein, Aspergillus niger, Agarose gel electrophoresis.
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