European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO STUDY OF IRON CHELATING ACTIVITY OF INDIAN WALNUT (JUGLANS REGIA) AND COFFEE (COFFEA ARABICA L.)IN VARIOUS EXTRACT.

Pallavi P. Lahane, Sangita S. Kunjwani, Zia H. Khan* and Nazia D. Khan

ABSTRACT

Metals with normal concentration have essential roles in body metabolism, however in higher concentration they can be induce sever toxicity. Treatment with chelating agents is useful practice to reduce metal toxicity in live organism. The fruit of Indian walnut (Juglans regia) and Coffee (Coffea arabica L.) were taken with the aim of evaluating the chelating activities. The extract in three different solvents viz. aqueous, methanol and petroleum ether were prepared. The chelating ability was studied by using Benzie and Strain method. EDTA was used as a standard for this purpose. Methanolic extract was found to be most effective iron chelator. Highest activity was found in 1000 ug/ml concentration of walnut plant i.e. 89.71% and coffee shows 90.32 % that of standard EDTA was found to be 97.90%. In conclusion the methanolic extract of Indian walnut (Juglans regia) and Coffee (Coffea arabica L.) could be used in treatment of iron overload diseases.

Keywords: Indian walnut (Juglans regia), Coffee (Coffea arabica L.), Iron chelating activity, EDTA.