European Journal of Biomedical and Pharmaceutical Sciences

ANTIMICROBIAL ACTIVITY OF Manihot esculanta ROOT METHANOLIC AND AQUEOUS EXTRACTS

Shama I. Y. Adam, Wasma A. A. Ahmed, Esra, O.M., Nazik,E. Y., Eiman.F. A, Shaimaa. A. A and Warda S. Abdelgadir

ABSTRACT

An experiment was conducted to assess the antimicrobial activity of methanolic and aqueous extract of Manihot esculanta root. Dried ground powdered bulb and peel of M. esculanta was evaluated against four Gram –ve bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonae and Salmonella typhimurium), one Gram +ve bacteria ( Staphylococcus aureus) and one yeast (Candida albicans) using the agar well diffusion method. At all concentrations (100, 50, 25 and 10%), the bulb methanolic extract exhibited antibacterial activity against all the test pathogenic bacteria with varying length of inhibition zones which were concentration dependant. K. pneumonae was found to be the most sensitive bacteria even to the peel methanolic extract at 100 and 50 %. C. albicans was found to be sensitive to both methanolic of the peel at concentration of 100% and aqueous extract of the bulb at 100 and 50%. These results were comparable with those produced by gentamicin (10μg) a reference antibiotic and nystatin (10mg/ml) a reference antifungal.

Keywords: Manihot esculenta, Antimicrobial activity, Staph. aureus, E. coli, Ps. aeruginosa,S. typhimurium, C. albicans.