IN VITRO ANTI- INFLAMMATORY ACTIVITY OF HEMIDESMUS INDICUS (ROOT) EXTRACT BY HRBC MEMBRANE STABILIZATION
Syed Sohail, Sangita Kunjwani, Zia. H. Khan and Shankar Mular
ABSTRACT
Hemidesmus indicus – Anantamool, is a plant species of Apocynaceae family commonly found in India, specially
in different areas of Maharashtra. It is a slender, laticiferous, semi-erect endangered shrub; specifically known for
its immense medicinal values; for example-anticancerous, antiarthritic, anti-inflammatory antimicrobial, antiulcer,
antivenom, antileprotic, immunomodulatory, hepatoprotective, wound healing activity etc. Its immense medicinal
values can bring H. indicus as a royal source of herbal medicine in India. Phytochemical constituents Literature
indicates the presence of Alkaloids, steroids, terpenoids, flavonoids, saponins, phenolic compounds, tannins and
lignins, inulins, cardiac glycosides, protein, carbohydrates etc., in aqueous and ethanolic hemidesmus indicus root
extract. Since triterpenoids and flavonoids have remarkable anti inflammatory activity, so our present work aims at
evaluating the in vitro anti inflammatory activity of Hemidesmus indicus by HRBC membrane stabilization. The
inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti inflammatory
activity. The percentage of membrane stabilisation for methanolic extracts and Diclofenac sodium were done at
different concentrations. The maximum membrane stabilization of Hemidesmus indicus extracts was found to be
79.64% at a dose of 50 μg/ml and Standard membrane stabilization was found to be 81.04% at a dose of 500
μg/ml of methanolic extract. Therefore, our studies support the isolation and the use of active constituents from
Hemidesmus indicus in treating inflammations.
Keywords: Indicus, Anti-inflammatory,Human Red Blood Cell (HRBC), Membrane Stabilization.
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