ANTICANCER ACTIVITY OF OIL OF THE SEEDS OF TRACHYSPERMUM AMMI
Gomathi Periyasamy, Ravinder Kashipeta
ABSTRACT
Trachyspermum ammi L. (Apiaceae) commonly known as ajwain is an important medicinal, aromatic and spice
plant. Ajwain seeds yield 2-5% brownish essential oil, consists of p-cymene (37.18%), γ-terpinene (35.36%) and
thymol (20.51%). Due to presence of numerous chemical constituents in ajwain, various biological and
pharmacological properties have been reported. In the present invention cytotoxic activity of volatile oil of T.
ammi was evaluated against three cancer cell lines viz., Human alveolar lung adenocarcinoma cell line (A549),
Human cervix adenocarcinoma cell line (HeLa) and Human gastric adenocarcinoma cell line (AGS) using MTT
assay method. The cell lines were treated with different concentrations of volatile oil (12.5, 25, 50, 100 and 200
ug/ml) and the cytotoxicity assay was done by MTT assay. The volatile oil showed effective toxicity
against A549, Hela and AGS cells with IC50 concentration of 26.78, 34.24 and 171.02 ug/ml respectively
after the incubation period of 24hrs. Cisplatin with 15ug/ml was used as a standard control for the study. The
results showed that the volatile oil may have satisfactory toxic potential against the tested cell lines.
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