European Journal of Biomedical and Pharmaceutical Sciences

MOLECULAR DETECTION OF QUINOLON RESISTANCE GENES IN CHLAMYDIA TRACHOMATIS ISOLATED FROM WOMEN WITH GENITAL INFECTIONS

Roya Torabizadeh*, Gita Eslami, Latif Ghachkar and Mehdi Goudarzi

ABSTRACT

Introduction and aims: Chlamydia trachomatis infection spread worldwide and causes differrent diseases. In many countries the most genital infection is chlamydia trachomatis. Tetracycline, macrolides and quinolones are common therapeutic options for treatment of infection. Resistance to quinolones in C. trachomatis clinical isolates has become an emerging challenge especially in women with genital infection. The aim of this study was to investigate the antimicrobial susceptibility patterns of C.trahomatis isolates resistance to quinolone agents and the mutation of gyr A and parC genes. With molecular methods in women with genital infection. Materials and methods During a 15 months study, 200 specimens were collected from patients with genital infection. Nested PCR was used for identification of C. trachomatis. Presence of gyrase (gyrA) and topoisomerase IV (parC) and mutation was identified using PCR assay. for identification of C. trachomatis. Presence of gyrase (gyrA) and topoisomerase IV (parC) and mutation was identified using PCR assay. we understand that there are 54 cases from 80 cases which are positive for having mutation in gyrA gene (67.5%).and 18 cases are positive for having mutation in parC gene. (22.5%). Result: Out of 200 specimens which had taken from women with vaginal discharge, D&C or abortion, 80 cases were positive for chlamydia trachomatis by Nested PCR. we understand that there are 54 cases from 80 cases which are positive for having mutation in gyrA gene (67.5%).and 18 cases are positive for having mutation in parC gene. (22.5%). we understand that there are 54 cases from 80 cases which are positive for having mutation in gyrA gene (67.5%).and 18 cases are positive for having mutation in parC gene. (22.5%). Our result revealed high resistance to quinolone among clinical chlamydia trachomatis isolated from patients, the percentage of mutation for both gyrA gene (67.5%)and parC gene (22.5%) were high, especially for gyr A gene, it seems that the second generation of quinolones are not effective drugs for treatment of chlamydial infections any more. Discussion: Our result revealed high resistance to quinolone among clinical chlamydia trachomatis isolated from patients, the percentage of mutation for both gyrA gene (67.5%) and parC gene (22.5%) were high, especially for gyr A gene, it seems that the second generation of quinolones are not effective drugs for treatment of chlamydial infections any more.

Keywords: Chlamydia trachomatis, quinolone resistance, gyr A gene, par C gene.