Abstract
PLATELET GLYCOPROTEIN IIB POLYMORPHISM AND PLATELET INDICES IN SUDANESE PATIENTS WITH SICKLE CELL ANEMIA

Shahnzad Abrha Hagous and Ibrahim Khider Ibrahim*

ABSTRACT

Background: Glycoprotein II b it is a receptor on platelets for fibrinogen and von will brand factor, and play vital role on platelet activation, aggregation and adhesion to sub endothelial .glycoprotein found in chromosome 17. Furthermore, any defect in this Glycoprotein II b might be a cause for many thrombotic disease has been reviewed in literature. Objective: The study aimed to investigate the frequency of the Glycoprotein II b polymorphism, platelet count and platelet indices in Sudanese patients with sickle cell anemia. Material and Methods: 2.5 ml of EDTA anticoagulated veinous blood was taken from 44 patients with Sickle cell anemia have been referring to Omdurman hospital, during May 2015-July 2015.Then, platelets count and platelets indices were performed by full automated hematological analyzer (Sysmex –KX21N, Japan). Genomic DNA extracted from whole blood samples by using DNA extraction kit (Intron–Korea).The platelet Glycoprotein II b polymorphism at position 843 (Lsolleucine/Serine) by using RFLP-PCR. Result: This study is a case control study, the most genotype frequency for patients were (Ile, Ser) 22(50%) followed by (Ile, Ile) 17(38.6%) and (Ser, Ser) 5(11.4%); while, the most frequency genotypes of control group were (Ile, Ile) 27(61.4%) followed by (Ile, Ser) 12 (27.3%) and (Ser, Ser) 5(11.4%).There were statistical significant between case and control for genotypes (Ile, Ile); (OR: 2.522 ; CI: (1.069-5.950), P.V:0.03), and (Ile, ser) (OR:0.375 ; CI:(0.154-0.912), P.V:0.029 ); but genotype (ser ,ser) showed statistical insignificant for both case and control (OR:1.0 ; CI:(0.268-3.731), P.V:1.0 ).There were no association between platelets indices (PDW, MPV and P-LCR) and platelets count with genotypes (P value :0.372, 0.758 , 0.146 and 0.414) respectively. The allele frequency for control was isolleusine :0.75 , serine :0.25 .While the allele frequency for the patients was isolleusine :0.64 , serine: 0.36 .However , a significant deviation from the Hardy- Weinberg equilibrium was observed in control group (X2=3.27, df=1 and p.v>0.05) and for patients group(X2=0.28 , df=1 and p.v: >0.05). Conclusion: The platelet glycoprotein IIB genotypes (Ile,Ile) and (Ile,Ser) might be consider the risk of sickle cell anemia complication.

Keywords: Sickle cell anemia, Platelets Polymorphism IIB, platelet indices.


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