COMPARATIVE EVALUATION OF THE CYTOTOXICITY OF BIODENTINE CEMENT MIXED WITH A NEW POTENTIAL REGENERATIVE SCAFFOLD HYDROGEL TO PERIODONTAL LIGAMENT FIBROBLAST CELLS.
Dr. Malini Shankar*, Dr. Preeti Dodwad, Dr. Kishore Bhatt, Dr. Nikhil Jambagi, Dr. Ravindra Singh
Shekhawat, Dr. Niraj Godbole
ABSTRACT
Aim of the study: To investigate the cytotoxicity of Biodentine (Septodont, France) cement mixed with a new
potential regenerative scaffold hydrogel (Placentrex) to periodontal ligament (PDL) fibroblast cells.
Methodology: Periodontal ligament fibroblast cells were seeded into 24 well plates containing Biodentine
(Group1), placentrex (Group 2), Biodentine coated with placentrex (Group 3) and Biodentine mixed with
Placentrex gel (Group 4) respectively, in 6 wells each for 7 days by changing the growth medium every other day.
On the 3rd day, and 7th day, the cell viability and proliferation of the cells was assayed using the MTT assay test
and the absorbance reading at 490nm was recorded in an ELISA plate reader. Results and Conclusion: Within
the limitations of the present study, Both Biodentine and Placentrex are highly biocompatible materials Biodentine
coated with Placentrex gel was least cytotoxic and showed increased proliferation of cells. The use of Placentrex
gel as an injectable scaffold has immense potential in regenerative procedures.
Keywords: Biodentine cement, Placentrex gel, cytotoxicity.
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