IN-VITRO EVALUATION OF ANTI INFLAMMATORY ACTIVITITY OF ETHANOLIC EXTRACT OF TRITICUM AESTIVUM (WHEAT GRASS)
Tirupathi Rao Annavarapu*, Samidala Nageswara Rao and Kaladi Hari Kamesh Kiran
ABSTRACT
The present investigation was carried out to evaluate the anti-inflammatory potential of solvent extracts of
Triticum aestivum (wheat grass). The anti-inflammatory activities were assessed through in vitro, the results
were found to be very surprising and promising. Ethanol solvent extracts of Triticum aestivum (wheat grass) were
found to have significant anti-inflammatory activity. The ethanolic fractions of the plant causes significant
reduction in inflammation compared to standard anti-inflammatory drug, diclofenac Sodium. Thus results showed
that extracts showed significant anti-inflammatory activity in dose-dependent manner. The extracts exhibited
membrane stabilization effect by inhibiting hypotonicity induced lysis of erythrocyte membrane. The erythrocyte
membrane is analogous to the lysosomal membrane, and its stabilization implies that the extract may as well
stabilize lysosomal membrane. Stabilization of lysosomal membrane is important in limiting the inflammatory
response by preventing the release of lysosomal constituents of activated Neutrophils such as bacterial enzymes
and proteases which cause further tissue inflammation and damage. Protein denaturation is also used to assess to
anti-inflammatory activity of wheat grass. The results show that decrease in protein denaturation is presented by
increase in absorbance. The present investigation indicate that wheat grass possess anti-inflammatory activity.
Keywords: Triticum aestivum (wheat grass), anti-inflammatory, HRBC, Protein Denaturation.
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