Abstract
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CARBAMAZEPINE IN FORMULATED PRODUCT USING REVERSE PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (RP-UPLC)

Chhama Shukla*, Majee Chandana and Sanchit Srivastav

ABSTRACT

The novel method was performed to develop and validate a rapid and selective analytical method by using Reverse Phase Ultra performance Liquid Chromatography (RP-UPLC) technique for the analysis of carbamazepine in raw materials & their pharmaceutical dosage forms. The developed analytical UPLC method is superior in technology to conventional HPLC method with respect to speed, resolution, solvent consumption and cost of analysis. The compound was analyzed with a total run time of 2.5min. (In reverse phase) at 237 nm wavelength. Optimum retention was achieved on Waters Acquity UPLC BEH C18 column (2.1 × 30mm, 1.7μm) using gradient elution with mobile phase i.e. ACN: H2O (50:50). The method showed excellent recoveries for all drugs in bulk. The developed UPLC method was validated with respect to specificity, linearity, precision, accuracy, ruggedness (reproducibility), robustness and stability. The method is economical in terms of the time taken and the amount of solvent used, thus promoting green chemistry concept. To the best of our knowledge, a work on method development and validation of Carbamazepine by using RP-UPLC technique, disclosed in this investigation, was not published elsewhere.

Keywords: Carbamazepine, UPLC, Accuracy, Precision, Robustness.


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