Abstract
IDENTIFICATION OF QUERCETIN PRESENT IN ETHANOL LEAF EXTRACT OF AMARANTHUS SPINOSUS BY CHROMATOGRAPHIC TECHNIQUES

S. Asha*, R. Rekha and A. Mohamed Sadiq

ABSTRACT

To establish the analytical issues for simultaneous determination of different phytochemicals present in the Amaranthus spinosus. Phytochemical tests were carried out in the present investigation. Further, a rapid and simple thin-layer chromatography, high-performance thin-layer chromatographic (HPTLC) and high pressure liquid chromatography method was developed for the qualitative and quantitative analysis. Preliminary phytochemical analysis revealed the presence of carbohydrates, protein, glycosides, flavonoids, phenol, steroids, alkaloids and absence of saponin and tannin. Toluene: ethyl acetate: formic acid (5:4:0.2) was used as a mobile phase for the identification of quercetin in the Amaranthus spinosus extract. HPLC analysis revealed 0.002% of quercetin in the ethanol extract. These results may be useful in search of plant – based pharmaceutical preparation to treat various disorders.

Keywords: TLC, HPTLC, HPLC, Quercetin.


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