IN VITRO ANTHELMINTIC EFFECT OF ETHANOLIC AND AQUEOUS EXTRACTS OF CALOTROPIS PROCERA, AZADIRACHTA INDICA AND PUNICA GRANATUM ON TRICHURIS GLOBULOSA, AN INTESTINAL NEMATODE OF SHEEP
Rama Aggarwal, Mansi Suri and *Upma Bagai
ABSTRACT
Indigenous system of herbal therapy is becoming an increasingly attractive approach to control parasitic infection
particularly in developing countries. Among parasites, helminth infections are responsible for considerable losses
to the livestock industry of marginal farmers. The aim of the present study was to evaluate anthelmintic effect of
both ethanolic and aqueous extracts of Calotropis procera flowers, Azadirachta indica leaves and Punica
granatum fruit peel in comparison with albendazole on Trichuris globulosa a nematode, parasitizing the large
intestine of sheep, through in vitro studies by the worm motility inhibition assay. LC-50 values were determined
to be 4.21 mg/ml ± 1.26 and 4.77 mg/ ml ± 1.00 for C. procera, 5.85 mg/ml ± 1.38 and 7.49 mg/ml ±1.98 for A.
indica, 9.56 mg/ml ± 2.78 and 8.75mg/ml ± 2.00 for P. granatum, ethanolic and aqueous extracts respectively,
whereas it was 10.73μg/ ml ± 2.88 for albendazole. The mean mortality index (MI) was 0.83 and 0.33 for C.
procera, 0.66 and 0.5 for both A. indica and P. granatum, ethanolic and aqueous extracts respectively whereas for
albendazole it was 1.0. Percent mean worm motility inhibition (%WMI) was observed to be between 45.8 and 100
% for different extracts after the worms were given lukewarm PBS treatment for 30 min after exposure to different
treatments for 3 h. It is concluded that all the three plants possess significant anthelmintic activity and could be
potential alternative for treating cases of helminth infection in ruminants.
Keywords: Anthelmintic Calotropis procera, Azadirachta indica, Punica granatum Trichuris globulosa, nematode.
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