PRE-ANALYTICAL INSURANCE OF HYPERLIPIDEMIC SAMPLE FOR D-DIMER ASSAY IN DIABETIC PATIENTS
Shireen Zuhdi Nimer and Hiba Badr Eldin Khalil*
ABSTRACT
Background; Lipid abnormalities are commonly associated with diabetes, particularly in those with type2
diabetes. The most common lipid abnormalities in these patients include hypertriglyceridemia and high cholesterol
(reduced high-density lipoprotein (HDL) and high (LDL).[4] In patients with diabetes, coronary artery disease is
the most common cause of death due to strong relationship between all forms of vascular disease in patients with
type 2 diabetes and hyperlipidemia.[5] Lipemia besides pathophysiological conditions, preanalytical laboratory
errors account for a large proportion of lipemic samples. Several mechanisms causing lipemia interference in
laboratory testing. Lipemia effect on coagulation test , and may falsely increase the D-dimer level.[9] This
study is aimed to compare the reality of D-dimer results after performed the pre-analytical insurance of
hyperlipidemic sample in Diabetic patients. Materials and Methods; The study was recruited 50 patients with
Diabetes mellitus type2 and diagnosed by hyperlipidemia A 2.5 ml citrated venous blood sample was
collected from each patient for classic centrifugation (protocol 1) and ultra-centrifugation (protocol 2).The Ddimer
level was measured by quantitative immunoassay using I-CHROMATM kit and reader. Results and
conclusions; Ultra centrifugation protocol for hyperlipidemic samples should be performed as pre-analytical
insurance before D-dimer assay. There were significant associations between high level of D-dimer and increased
level or abnormality of Lipid profile.
Keywords: D-dimer, Diabetic, Hyperlipidemia, Ultracentrifugation.
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