European Journal of Biomedical and Pharmaceutical Sciences

ASSOCIATION OF HUMAN TELOMERASE REVERSE TRANSCRIPTASE (HTERT) MNS16A POLYMORPHISM WITH HAEMATOLOGICAL FINDINGS LYMPHOID LEUKAEMIA IN SUDANESE PATIENTS

Omnia Noreldeen Gamaleldeen Mohamed and Ibrahim Khider Ibrahim*

ABSTRACT

Recently A minisatlite tanden repeat (MNS16A) located in the dounstream of the human telomerase reverse transcriptase (hTERT) gene has been identified and reported to have effect on the telomerase activity and Htert expression. The purpose of this study was to investigate the association between hTERT tandem repeat variants and hematological findings in lymphoid leukaemias. A total of 56 Sudanese patients with lymphoid leukaemias. 36 CLL and 20 ALL and 50 healthy volunteers acontrol group were enrolled in this study. Five (ml) of venous blood sample was collected from each subject and poured into ethylene diamine tetra acetic acid (EDTA) container for Complete blood count using automated Haematolgy analyzer and molecular analysis. DNA was extracted from anticoagulated blood sample using salting out method and the hTERT MNS16A variants were detected using allele specific polymerase chain reaction (PCR). The results showed that, there were 2 alleles in lymphoid leukemia's patients and also in the control group, these are 271bp and 302bp. Accordingly three genotypes were reported in CLL and ALL patients 271/271, 271/302 and 302/302, while only one genotype was reported in the control group 271/302 ; and there was no statistically significant association between hTERT MNS16A genotypic variants and difference in mean haematological findings of patients with lymphoid leukaemias.

Keywords: minisatlite tanden, dounstream, leukaemias.