European Journal of Biomedical and Pharmaceutical Sciences

GENETIC DIVERSITY ANALYSIS OF SELAGINELLA BRYOPTERIS (L.)BAK. GENOTYPES IN TELANGANA, INDIA.

*P. Rupa and N. Lakshmibhavani

ABSTRACT

The present study is aimed to evaluate the genetic diversity among the eight genotypes of Selaginella bryopteris (L.)Bak. belonging to Telangana state, India. Genetic variation among the genotypes was analyzed with PCR based Random amplified polymorphic DNA (RAPD) markers.12 RAPD primers OPA-01, OPA-02, OPA-03, OPA-04, OPA-05, OPA-09, OPA-10, OPA-13, OPA-16, OPA-19, OPC-05 and OPC-10 were used in this study. Amplified DNA fragments were separated in 1.5% agarose gel for RAPD analysis. A total 443 DNA fragments were amplified with an average of 6.81 RAPD markers per primer. A maximum of 9 fragments were amplified with primer OPC-05 and a minimum of 4 bands with primer OPA-09.The PIC ranged from 0.06-0.672. The dendrogram based on similarity coefficient showed two major clusters. Cluster-I consisted of Medak-1 and Medak-2. Cluster-II is divided into four sub classes including the remaining six genotypes. Maximum similarity was observed between RR-1 and RR-2 genotypes with 0.85% similarity.

Keywords: Selaginella bryopteris (L.)Bak.,Telangana., Genetic diversity and RAPD markers.