European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO PLANTLET REGENERATION FROM COTYLEDONARY EXPLANTS IN SOLANUM TORVUM (SWARTZ). A MEDICINALLY IMPORTANT PLANT

Thirunahari Ugandhar*

ABSTRACT

In vitro culture of medicinal plants is important for the production of plant based high quality medicine. Solanum torvum (Swartz) possess immense medicinal importance and in vitro culture for commercial production was achieved by culturing the plant using different concentration and combination of plant growth regulators. Mainly, the concentration and combination of auxins and cytokinins in the nutrient MS medium is the key factor which determines successful plant regeneration. In vitro plantlet regeneration has been obtained from 15-20days old seedling cotyledon segments of Solanum torvum (Swartz) were examined using various phytoharmons individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP/Kn/TDZ (1.0-5.0 mg/L) IAA (0.5 mg/L)+ BAP/Kn/TDZ (1.0-5.0 mg/L) and IAA (0.5 mg/L) for shoot proliferation IAA (0.5 mg/L)+BAP (3.0 mg/L) was proved to be best for induction of shoots for Cotyledon explants. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (1.0mg/L–5.0mg/L) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 86%. Plants looked healthy with no visually detectable phenotypic variations.

Keywords: Solanum torvum (Swartz), Plantlet formation, In vitro rooting shoot formation.