PHYSICO-CHEMICAL ANALYSIS, ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA FROM DRINKING WATER IN GUMMA PANCHAYATH, VISAKHAPATNAM, ANDHRA PRADESH.
Laxmi Sowmya, K., *Sandhya Deepika, D. and N.S.M Jyothi
ABSTRACT
The present study was undertaken to evaluate the water quality of the Gumma panchayat of Ananthagiri mandal in Visakhapatnam district with affable means. The physicochemical and the microbial studies are most important regions by which we are able to test the portability of water. The isolation and characterization of the pathogenic microorganism from the water sample collected were the main emphasized area of the study. In this study drinking water samples were collected from a hand bore and a stream for a period of one year i.e., from April 2011 to March 2012. The various constituents monitored include the physicochemical characters, the bacterial parameters like Total plate count (TPC), Most probable number (MPN) and isolation and identification of pathogenic bacteria. The physicochemical characters of all the two drinking water samples were with in the recommended permissible level of WHO. The total plate count was above the WHO guidelines values (<10CFU’s/ml) in the Two water samples studied and the highest count was during August and September. The bacteria isolated were E. coli, Salmonella, Shigella, Staphylococcus, Group D Streptococcus, Vibrio cholera and V. parahaemolyticus and Klebsiella pneumoniae. The samples were inoculated and were incubated at 370C for 24 hrs or 48hrs.for appropriate bacterial growths. Thus we can use this study for the assessment of the water and to resolve the hygienic problems of the water.
Keywords: Drinking water, Quality assessment, pathogenic bacteria, Gumma panchayat.
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