Abstract
ISOLATION, PURIFICATION AND CHARACTERIZATION OF PECTINASE

Nithisha K. T., Lavanya S., Shivaji Bole* and Mahesh M.

ABSTRACT

Pectinases are the enzymes which breakdown pectin polysaccharide present in plant tissues into simpler molecules like sugar and other useful compounds. Pectinases account for 10% of the total worldwide production of enzymes. In the present study, bacteria were isolated from soil sample and screened for the production and characterization of pectinase. The bacterial isolate was identified as Bacillus sp. Maximum yield of poectinase was obtained after 72 hrs incubation., The enzyme activity was performed by titrimetric method using starch as an indicator and polygalactouranase as substrate, The maximum enzyme activity was shown as 40 units/ml, The Optimum temperature and pH for enzyme activity was found to be 35°C, pH-6.0 respectively. The induced stress showed maximum activity at 30mg of salt concentration and the molecular weight of the protein was found to be 40kDa.

Keywords: Bacillus sp., Polygalactouronase, Pectinase, Temperature, pH, Stress.


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