Abstract
CHEMICAL CONSTITUENTS AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF THE LEAVES OF CITRUS LIMON (L.) BURM. F.

Shahnaz Sultana*, Andleeb Khan, Farah Islam, Fakhrul Islam, Ibrahim A. Khardali, Mohsen M. Fageeh, Hassan A. Alhazmi and Mohd. M. Safhi

ABSTRACT

Citrus limon (L. ) Burm.f. (Rutaceae) is a small evergreen tree found in Asia, Mexico and South Africa. Its leaves are sedative, antispasmodic and antiseptic; used to treat insomnia, nervousness, palpitation, migraine headache and asthma. GC-MS analysis of an ethanolic extract of the leaves indicated that the predominent constitutents of the extract were n-tetracontane (9.07) followed by n- hexacosane (5.88%), 9-octadecenamide (4.60%), 5,7-dimethoxy-2H-1-benzopyran-2-one (4.06%), n-hexatriacontane (3.11%), methoxsalen (2.61%), n-dotriacontane (2.37%), n-hexadecanoic acid (2.36%), tetradecanamide (2.14%) and stigmast-5-en-3β-ol (2.04%). Fifty five compounds were present in traces. The leaf ethanolic extract showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Streptococcus pyogenes.

Keywords: Citrus limon, leaves, ethanolic extract, chemical composition, antimicrobial activity.


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