European Journal of Biomedical and Pharmaceutical Sciences

THE EFFECT OF GINGER TINCTURE AND OLIVE OIL ON THE EXPERIMENTALLY INDUCED BONE MARROW DEPRESSION IN ADULT MALE ALBINO RAT

Mai MH Selim, Rehab A. Hasan* and Mona AA Arafa

ABSTRACT

Background: Cyclophosphamide (CP) is a nitrogen alkylating agent used in various types of cancer chemotherapy, in spite of that CP chemotherapy induces sever bone marrow damage and depression. Many natural compounds derived from plants proved to have a protective effect against the toxic effects of many chemicals, such as ginger plant which is considered as a rich source of antioxidants, while olive oil plays a well established, beneficial role in promoting good health. Material and method: Forty adult male albino rats were divided into four equal groups: GI; control group, GII; ginger and olive oil receiving group, GIII; CP injected group, GIV; group receiving both CP with ginger and olive oil. This experiment continued for 15 days. At its end blood samples were collected for complete blood picture and bone marrow samples were taken for histological, immunohistochemical and chromosomal studies. Morphometric and statistical studies were also done. Results: CP caused prominent deficiency in the hemopoietic cells within the bone marrow, it decreased the RBCs, WBCs and platelets count in peripheral blood. CP produced depression of dividing bone marrow cells. This was detected by PCNA immunohistochemical study. Also, it produced some chromosomal structural changes. All these changes were improved when CP given in association with ginger tincture and olive oil. Conclusion: Administration of ginger tincture and olive oil is playing a role in protection of the body against the harmful effects of some toxic agent as CP.

Keywords: cyclphosphamide, ginger, olive oil, bone marrow and chromosomes.