European Journal of Biomedical and Pharmaceutical Sciences

OPTIMISATION AND DOWNSTREAM PROCESSING OF AMYLASE SYNTHESIZED FROM MARINE ACTINOMYCETES- NOCARDIOPSIS ALBA AND ITS DESTAINING PROPERTY.

P. Sandhya, S. Yamini Sudha Lakshmi*, K. Saraswathi and P. Arumugam

ABSTRACT

Marine actinomycetes have been a well known source of many biological products. In the marine environment, marine microorganisms are the eminent source to discover new bioactive compounds. Among marine microorganisms, the actinomycetes are the major source for the production of new drugs which is proven by the various earlier researches. Actinomycetes are a group of prokaryotic gram positive filamentous bacteria which produce two types of branching mycelium, aerial mycelium and substrate mycelium. Some actinomycetes genera are slow growing microorganism. They belongs to the domain bacteria, phylum actinobacteria, subclass actinobacteridae, order actinomycetales, suborder actinomycineae, family actinomycetaceae. The member of this order contains high G+C content (>55 mol%) in their DNA. The present work is concerned about the economic and efficient production of amylase enzyme from Nocardiopsis alba. The sand sample was collected from Thiruvanmiyur Beach. The sand sample was used for screening and isolation of Nocardiopsis alba. The pure culture isolated was screened for its enzyme activity. The species showed positive results for Iodine test. The enzyme optimization using various parameters like pH ranging from 7.2 to 7.8, temperature- 4°C, 35°C, 37°C & 65°C with the media (ISP 1-7) was carried out. This was followed by the downstream processing of the enzyme and checked for the enzyme purity. The purified enzyme was then subjected to destaining activity as one of its applications.

Keywords: Marine Actinomycetes, Nocardiopsis alba, Amylase, Optimisation, Downstream Process and Destaining property.