NANOSILVER SYNTHESIS USING A GRAM NEGATIVE PSEUDOMONAS AERUGINOSA (MTCC 2453) AND ITS POTENTIAL APPLICATION ON URINARY TRACT INFECTING BACTERIA
P. Prema*, N. Subashini, M. Nageswari and N. Alagumanikumaran
ABSTRACT
The use of gram negative bacteria like Pseudomonas aeruginosa in the synthesis of nanosilver emerges as an ecofriendly approach and an alternative to the chemical method. In the present attempt, the microbial mediated synthesis of silver nanoparticle (AgNPs) using a gram negative bacteria, Pseudomonas aeruginosa. Silver nanoparticles were synthesized Ag+ ion using the bacterial culture supernatants at room temperature. Synthesized nanosilver was observed visually by color change from pale yellow to brown which was confirmed by UV-vis spectroscopy. The AgNps were further characterized by FTIR, SEM, XRD and AFM analyses. The AgNps were found to be spherical in shape with a size in the range of 10-20nm. The synthesized nanosilver were found to have an antibacterial activity against four selected urinary tract infecting pathogenic bacteria such as Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Proteus vulgaris. Thus, the biosynthesis of silver nanoparticles using a gram negative Pseudomonas aeruginosa deserves to be a good candidate as an antibacterial agent for UTI pathogens.
Keywords: AgNPs, FTIR, XRD, SEM, AFM and UTI pathogens.
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