HPTLC ANALYSIS OF BERBERINE FROM TINOSPORA CORDIFOLIA STEM EXTRACT
Shivam Singh*, Pragya Singh, Abhinai Singh, Arvind Tripathi, Shrikant Kaul, Riya Shrivastava, R. M. Mishra
ABSTRACT
Estimation of Berberine from High-performance thin layer chromatographic method was developed for ancient Indian medicinal climber Tinospora cordifolia based on its specificity and accuracy. The shed dried stem of Tinospora cordifolia was subjected to Methanolic extraction with a good yield of 4.98 (W/W). A TLC aluminium sheet precoated with silica gel 60 F254 was used as the stationary phase. The mobile phase system consisting of Toluene: Ethyl acetate at ratio 9:3 (v/v) with Rf value of 0.05 produces a good resolution of Berberine. Scanning analysis was performed at an absorbance wavelength of 266 nm. The calibration graph of Berberine was obtained in the range of 1.0 to 7.0 μL/spot and exhibit appropriate correlation coefficient 0.9994. The intra-day and inter-day precision analysis of Berberine was found to be 0.32 and 0.38, respectively. The concentration of Berberine in the stem of Tinospora cordifolia with respect to dry weight basis was found to be 0.945 mg (w/w) with Mean % RSD of 1.41. Statistical investigations proved that the proposed process is precise and reproducible. The anticipated method was also helpful for the assessment of Berberine from crude medicinal formulations containing Tinospora cordifolia.
Keywords: Tinospora cordifolia, Berberine, Methanolic.
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