DETECTION OF THE PLATELETS RECEPTOR GPIIB POLYMORPHISM IN SUDANESE PATIENT WITH POLYCYTHAEMIA VERA.
Nafisa Abdel Magid Ahmed Mohamed and Ibrahim Khider Ibrahim*
ABSTRACT
Background: Glycoprotein IIb it is a receptor on platelets for fibrinogen and von will brand factor and aid in platelet activation, aggregation and adhesion to sub endothelial .glycoprotein found in chromosome 17 and defect in it will cause many diseases. Objective: The purpose of this study was to investigate the frequency of the GPIIb polymorphism carried out among polycythemic patientspatients patientspatients and healthy individuals and healthy individualsand healthy individualsand healthy individualsand healthy individuals and healthy individuals and healthy individualsand healthy individualsand healthy individualsand healthy individualsand healthy individualsand healthy individualsand healthy individuals and healthy individuals and healthy individualsand healthy individuals and healthy individuals in Sudan. Material and Methods: 44 polycthemic patients and 44 controls were evaluated to detect the frequency of GPIIb polymorphism. Platelet parameters were performed by an automated cell analyzer. The GPIIb polymorphism was detected using RFLP-PCR method. Result: The wild genotype for GPIIb were detected in 17 of patients and 27 of the control; the heterozygous genotype were detected in 18 of patients and 12 of control and the homozygous genotype were detected in 9 of patients and 5 for control and the difference was not statistically significant (P. value-0.065). Conclusion: the allele frequency for control was: serine=0.75, isolleusine=0.25.while the allele frequency for the patients was: serine =0.41, isolleusine=0.59. However, a significant deviation from the Hardy- Weinberg equilibrium was observed in control group (X2=3.27, df=1 and p.v>0.05) while for patients group was insignificant (X2= 0.03, df=2 and p.v =0.98).
Keywords: polycythemia vera, Platelets, Polymorphism, GPIIB.
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